Gene isolation
The isolation of genes is of increasing importance for industry. Plant Research International has several advanced technologies at its disposal. The methods range from 'chemical analysis' such as the ultra high throughput DNA sequencing, to functional analysis such as with the gene machine.

• DNA Sequencing: Ultra high throughput sequencing
• DNA Chip technology: Gene expression and detection centre
• Gene Machine: arabidopsis population, saturated with transposons
• Ideotyping with simulation models, for improvement of plant traits by breeding and genetic modification (e.g., yield enhancement, quality improvement, resistance, and in combination with agro-ecological characterization).
• Technology assessment of genetically modified plants (e.g., analysis of plant physiological side effects, ecological risks, with use of specialised facilities Rhizo- and Phytolab).
• Genes: Plant Research International has identified and developed many genes which can be of use in research and plant breeding. Examples of identified genes are the homeotic genes involved in flowerformation (ADS-box genes) and the gene involved in flavour development in strawberry.Plant Research International has a patent on the construction of so called hybrid Bt-toxins. With this technology, toxins can be constructed with a new activity spectrum and higher activity.

Reports on biosafety

Plant Research International carries out studies into the biosafety of transgenic plants by order of the Dutch government. The reports are being used for policy development and are available upon request (info.plant@wur.nl).

Titles of published reports on biosafety:

• A transgene-centred evaluation of genetically modified plants
  • Part 1: Overview of selection and reporter genes
  • Part 2: Biosafety of genetically modified phosphinotricin-tolerant plants
  • Part 3: Biosafety of genetically modified glyphosate-tolerant plants
  • Part 4: Biosafety of ALS-herbicide tolerance-conferring (ALSHT) genes in plants
  • Part 5: Biosafety of E-coli-â- glucuronidase in plants
• Assessment of allergenic potential of foods from genetically modified plants
• Kanamycine resistentie in transgene planten (Dutch)