Technology
The DArT genotyping technology consists of several steps:

• Cutting DNA from reference organism(s) and amplification of a set of restriction fragments
• Generation of a library of these restriction fragments
• Printing the library on glass slides, giving micro-arrays
• Cutting DNA from target organisms and amplification of a set of restriction fragments
• Adhering fluorescent labels to these fragments
• Hybridization of the labeled fragments onto the micro-arrays
• Scanning
• Data analysis

Differences in hybridization patterns of target organisms reflect genetic differences between these organisms.

Equipment
Plant Research International has a good infrastructure for performing DArT such as micro-array facilities and colony picker and experienced highly qualified staff. Plant Research International developed a flexible, highly reproducible method for generation of sets of amplified restriction fragments for DArT.