Lab protocols Potato late blight

Basic Techniques

• Isolation of field isolates from foliar lesions
• Culture media
• Mating type test
• Metalaxyl resistance test
• Storage in liquid nitrogen
• Laboratory manual for P. infestans work at CIP-Quito, July 1997  (Source: CIP)

Oospore epidemiology

• Oospore production in leaves
• Extraction of oospores from infected leaves
• Oospore production on artificial (agar based) media
• Assessment of oospore viability

Molecular characterization

• Genomic (total) DNA extraction from mycelium
• General protocol for PCR amplification
• AFLP protocol for ALFexpress