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Antisera and conjugates with fluorescent or enzymatic labels that are not listed can be produced upon request. Please inquire.
Characterization of plant-pathogenic bacteria by Biolog
Several reliable methods are available for characterization of unknown bacteria. One of these methods is based on the ability of a microorganism to utilize or oxidize specific carbon sources. In Biolog a 96 well microplate is filled with a number of nutrients and biochemicals containing different carbon sources and tetrazolium violet. The tetrazolium violet is used as a redox dye to indicate the utilization of a certain carbon source. The test yields a characteristic pattern of purple wells, by which bacteria can be characterized.
Characterization of plant-pathogenic bacteria by 16S rRNA analysis
Ribosomal RNAs occur in all organisms and consist of highly conserved regions. Comparative sequencing of the rRNAs has become a powerful tool in the search for relationships between organisms. One of the largest available data sets of complete sequences is for the small subunit ribosomal RNA (16S rRNA). Comparison of the 16S rRNA sequences has proved to be a reliable tool in bacteria characterization.
Parts of the rRNA of an unknown bacterium are amplified by using universal primers that are complementary to virtually all conserved 16s rRNA regions. The amplification product is sequenced and for comparison submitted to a large nucleotide database like GenBank ®. The identity of the bacteria is determined by searching the database for matches of highly similar sequences.
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